ABSTRACT
<p><b>OBJECTIVE</b>To improve the method of indirubin in serum by HPLC and apply to pharmacokinetics in rats.</p><p><b>METHOD</b>Chromatographic separation was conducted on an C18 column (4.6 mm x 250 mm, 5 microm), using a mixture of methanol-water (75:25) as mobile phase at a flow rate of 1.0 mL min(-1) with UV detection at 289 nm, the column temperature was at 35 degrees C and ethinyl estradiol was used as an internal standard. Rats were administered i. v. bolus of indirubin in doses of 2.0 and 4.0 mg x kg(-1) through a jugular vein catheter, respectively. Serial blood samples (about 100 microL) were individually collected at 2, 5, 10, 20, 30, 60, 90, 120, 180 min after administration, and the concentrations of indirubin determined were in rat serum by HPLC. The pharmacokinetic parameters were calculated with the Winnonlin 5.0 software.</p><p><b>RESULT</b>The calibration curve for indirubin was linear ( R2 = 0.9996) in the range of 0.031-2.48 mg x L(-1) and the limit of detection (LOD) was 31 microg x L(-1). The average recovery of indirubin in rat serum was more than 98% and the relative standard deviations of intra-day and inter-day were both less than 10%. The pharmacokinetics of Indirubin in rats was fitted to two-compartment model.</p><p><b>CONCLUSION</b>The method is simple and accurate with a high sensitivity and a good repeatability, and it can be applied to the evaluation of pharmacokinetic parameters of indirubin in rats and blood concentration of indirubin in clinical controlling.</p>
Subject(s)
Animals , Male , Rats , Chromatography, High Pressure Liquid , Methods , Indoles , Blood , Pharmacokinetics , Rats, WistarABSTRACT
Objective To explore the epidemic characters of Pathological Internet Usage (PIU) in the newly enrolled post-graduates and further to detect the relationship between PIU and relative social environment, etc., so as to provide more evidence for the etiology of PIU. Methods Questionnaire was conducted in 556 newly enrolled post-graduate students of a medical university. The recovery rate of the valid questionnaires was 94.72%, and the data were analyzed by chi-square test, t-test and logistic regression. Results The rate of internet-utilizing students in recent one year was 95.3%, thereinto, 5.3% of them was with internet addiction disorder. Multivariate logistic regression analysis showed that the major impact factors were gamble, playing games, the heavy study pressure, psychoticism personality characteristic tendency and low self-respect, the OR values (95%CI) were 13.966 (1.662~117.835), 3.440 (1.401~8.450), 1.249 (1.096~1.423), 0.889 (0.821~0.963) and 2.749 (1.167~6.475), respectively (all P
ABSTRACT
Objective:To explore the psychological risk factors of internet addiction disorder use(IAD) among the first grade graduate students of medical science,and to provide evidence for the prevention against IAD.Methods:Total 556 students completed a questionnaire including self-designed General Conditions Questionnaire,Symptom Checklist-90(SCL-90),Self-esteem Scale(SES),Eysenck Personality Questionnaire(EPQ) and Chinese Internet Addiction Scale-Revised(CIAS-R).The recovery rate of the valid questionnaires was 94.72%.Logistic multivariable analysis was used to find out the psychological risk factors.Results: One-factor analysis shows that significant related factors that emerged as common correlates with IAD were sex,present live-condition approval,study pressure,the level of mental health condition,personality characteristic,self-esteem etc.Multiple logistic regression analysis showed that the major impact factors were the heaver study pressure,psychiatric personality traits tendency and low self-esteem.Conclusion:the graduates IAD is closely connected with psychological factors.The relevant departments should pay much attention to their mental health,and guide them to use internet reasonably to avoid affecting mental and body health.